Special Ion Exchange Resin for Heparin Sodium Extraction


KPA-D654 is a premium grade macroporous strongly basic anion exchange resin of type I. This resin is polymerized by styrene crosslinking divinylbenzene, containing trimethylamine groups, which is ion exchanged with quaternary ammonium salt as the active functional group.

Attributed to its special macroporous structure(pore diameter, pore volume, specific surface area, etc) parameters right fit for the large molecular weight and size of heparin, D654 is featured as strong adsorption capability, high exchange velocity &large exchange capacity to heparin sodium, easy elution, stable physical and chemical properties, great mechanical strength, resistance to organic poisoning and long service life.

Overall, KPA-D654 is suitable for salt technology, especially for the extraction of heparin sodium by ion exchange method in the biomedical engineering industry. Its wet resin has been tested to exert high exchange capacity and rate for the residual heparin in the hydrolysate after salt hydrolysis and dissociation of fresh intestinal mucosa.

Matrix: Styrene-divinylbenzene Copolymer
Physical form: Creamy white to yellowish opaque spherical beads
Active functional groups: Quaternary ammonium salt
[ R-CH2-N+(CH3)3Cl ]
Moisture holding capacity(%): 75 to 85%
Ionic form as shipped: Cl
Total mass exchange capacity: ≥4.50mmol/g(dry)
Total bulk exchange capacity: ≥0.65mmol/ml
Moisture true density: 1.03~1.07g/ml
Moisture bulk density: 0.60~0.70g/ml
Particle size: (0.60~1.60mm)≥95%
Uniformity coefficient: ≤1.5
Integrated beads ratio after grinding test: ≥95℅
i. Pretreatment for Preparing Proto-Hydrolyzate:
Prepared hydrolysate solution
Porcine intestinal mucosa +5.5%NaCl
Operating temperature for hydrolyzate preparation : Kept at 50~55°C for 2hrs;
heated up to 95℃ holding for 10min
Applicable PH value of hydrolyzate: 9.0 ~ 9.5
ii. Resin Operating Conditions:
Operating temperature as resin is put into hydrolyzate for ion exchange : 50~60°C
Resin specific consumption: 3% hydrolyzate weight
Salinity scope during resin adsorption: 4.8~5.2°
IX adsorption duration: 5~6hrs(stirring adsorption)
iii. Post-treatment of Used Resins:
Rinse of resin: Rinse 2times with
7%NaCl; 0.5hr/time
Elution of resin: Elute twice with saturated saline solution
0.8times the weight of resin: 6~8hrs/1st; 6hrs/2nd.
During then, industrial liquid alkali can be added to adjust PH from 9.0 to 9.5, or heated to 500℃
Regeneration of resin for recycle

Rinse used resins several times with clear water;
After drainage, soak resins in saturated salt solution 24hrs;
Collect the brine that contains residual heparin;
Soak the regenerated resins in clear water for recycling use

iv. Post-treatment of Eluate for Extracting Refined Heparin
Precipitation of

Add 90~95% alcohol to eluate, adjusting alcohol content to 30~35° and salinity of eluate is under 15°;
Stir, sealed standing for 24hrs;
Adsorb the supernatant by siphonage method;
Extract the bottom sediments as (wet)crude heparin.
Dehydration of wet heparin:Add absolute ethanol to dried and crushed heparin for dehydration;
Repeated 2 to 3 times
Further Desiccation of heparin:
Place heparin in drying oven at 60~70℃
Re-precipitation of crude heparin:Dissolve, heat, boil and filter the mixture of 1kg crude heparin+10kg water +500g salt;
Add 40°alcohol to re-precipitate (wet) fine heparin
Refining:Re-dehydrate and re-desiccate to obtain (dry) refined heparin